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Journal: Molecular Biomedicine
Article Title: Curcumin alleviates metabolic dysfunction-associated steatotic liver disease via gut microbiota-dependent epigenetic alterations
doi: 10.1186/s43556-026-00606-1
Figure Lengend Snippet: C. sensu regulated lipid accumulation in hepatocytes in a CEBPB-dependent manner. a-d Representative BODIPY 493/503 fluorescence staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or control and transfected with siRNAs against CEBPB (si- CEBPB ) or negative control siRNA (si-NC) with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). e–f Quantification of intracellular TG content in HepG2 and Huh-7 cells treated with C. sensu or control transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM, n = 3). g-j Representative ORO staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or Control and transfected with si- CEBPB or si-NC, with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). k The levels of lipid transport- and synthesis-related proteins ( ACC1 , CD36 , and SCD1 ) in C. sensu - or control-treated HepG2 and Huh-7 cells transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM) were assessed by Western blotting ( n = 3). Results were presented as mean ± SD from three independent experiments. P values were calculated with one-way ANOVA followed by Tukey’s test. The abbreviation C. sensu represents Clostridium_sensu_stricto_1 . * P < 0.05, ** P < 0.01, *** P < 0.001
Article Snippet: The primary antibodies used were as follows: AHR (67785–1-Ig, Proteintech, China), CEBPB (23431–1-AP, Proteintech, China), ACC1 (21923–1-AP, Proteintech, China),
Techniques: Fluorescence, Staining, Analysis, Control, Transfection, Negative Control, Western Blot